Laser Scanning Confocal Microscopy - LSCM 1

 

Modular design

Compatible with a variety of microscopes

Customized service

Laser Scanning Confocal Microscopy - LSCM 1

Core Index of LSCM1

Laser

405nm、488nm、561nm,638nm,power≥20mW,can continuously adjust the laser intensity

Control method of laser

  Each laser switch and power adjustment can be controlled separately, the software can directly adjust all laser switches and the intensity

Scanning method

2 independent silver-plated XY scanning galvanometers

Scan speed

50Hz、100Hz、200Hz、400Hz、100lines/s to 600lines/s optional

Scan resolution

128×128、512×512、1024×1024、2048×2048

Lateral resolution  

220nm

Scan mode

XY、XYT、XYZ、XYZTetc.

Z-axis focusing

Scanning table equipped with piezoelectric objective lens focusing (travel≥300μm)

Detection mode

Filter optical splitting

Detection channel  

≤4 channel fluorescence detection, all channels can be scanned in real time and simultaneously superimposed

Detector

High sensitivity photomultiplier tube ( GaAsP) or Multi-alkali photomultiplier tube, the spectrum detection range is 400nm-700nm

IPC  

Windows10 64bit operating system

Microscope body

Inverted fluorescence microscope (equipped with optical plate)

Objective lens, eyepiece  

10X(NA 0.42)、20X(NA 0.75)、40X(NA 0.95)、100X(oil lens,NA 1.4)APO objective lens,pair of eyepieces (10X)

Confocal pinhole  

30μm(about 1AU)

Basic software features  

Graphic user interface, user setting interface, image processing interface, image acquisition and system automatic control function specially designed for dark environment

Image Processing  

2D image processing functions, including image brightness, contrast adjustment, zooming, cropping, measurement, scale, pseudo-color disassembly, etc. It has user setting interface, image processing interface, image acquisition, automatic control system , and a graphical user interface specially designed for the darkroom environment.

Image processing software  

3D image synthesis, observation, zoom, contrast, etc.

Main Functions of LSCM1

index
  • Preview: single-channel or multi-channel detection can be realized, and the imaging display can be performed at different speeds;

  • Multi-channel imaging: single-channel, multi-channel imaging and multi-channel fusion can be realized;

  • Multi-channel time-sharing imaging: multi-channel time-sharing imaging and multi-channel fusion can be realized;

  • Image saving: The scanned images can be saved in real time;

  • Crop function of scan field: could select the imaging area of interest for imaging;

  • Rotate function of scan field (manual);

  • Time series: Automatic imaging of the sample at a customized time interval;

  • Z Stack and time series: Automatic Z-axis tomography of the samples with customized time intervals;

  • Image processing.

The application case

Clients:

Kunming Institute of Zoology - Chinese Academy of Sciences

Other trial cases:

Institute of Neurology - Suzhou University Shanghai Institute of Materia Medica - Chinese Academy of Sciences Institute of Zoology - Chinese Academy of Sciences

 

Special advantages

Modular design:

Easy to install and maintain, the purchase of modules can directly upgrade the experimental microscope

Suitable for a variety of microscopes:

Nikon, Olympus, Yongxin, etc

Customized services:

Customization can be carried out according to customers' needs so as to meet special needs such as scientific research and clinical practice.

 

 

 

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